MTHFR基因多态性与血浆同型半胱氨酸在妊娠期高血压疾病的相关性研究

The research on the relationship between the polymorphism of MTHFR gene and HCY in patients with Hypertensive disorder complication pregnancy

目的探讨四氢叶酸还原酶(MTHFR)C677T基因多态性及血浆同型半胱氨酸(Hcy)在妊娠期高血压疾病(HDP)发病机制中的作用。方法用过柱法提取基因型DNA,并行聚合酶链反应物-直接测序法检测和分析MTHFR基因中C677T的分型,用微粒酶免疫发光法测量血浆同型半胱氨酸水平,结果①HDP组与对照组C677/T677比较差异有统计学意义(P<0.05),HDP组T677等位基因频率(0.488)显著高于对照组(0.325),两组比较差异有统计学意义(P<0.01)。②轻度子痫前期组、重度子痫前期组的血浆Hcy水平均高于对照组,分别比较,差异均有统计学差异(P<0.001)。结论①妊娠期高血压疾病患者的MTHFR C677T基因多态性可能是妊娠期高血压疾病发生的诱因之一,T667等位基因可能是妊娠高血压疾病的易感基因。②血浆Hcy水平升高可能是子痫前期发病的危险因素。

Objective： To investigate the role of Hcy and polymorphism of MTHFR gene （C677T） in the pathogenesy of HDP. Methods ： The genotype DNA was extracted by chromatographic column, and the typing of C677T was analyse by PCR - directly detection method. The Hcy was determined using microparthcle colormetric immunoassay method. Results： ① In HDP group, to C677/ T677 genotype, the difference is obvious （ P 〈0. 05）. T677 allele frequency was higher than that in control group （ P 〈0. 01 ）. ② The level of Hcy in mild preeclampsia group and severve preeclampsia significantly increased than that in control group （ P 〈0. 001 ）. Conclusion ： ①Polymorphism of MTHFR C677T gene may induce the HDP and T667 is regarded as predisposing genes to HDP. ②The increase of Hcy in plasma is the risk factor of preeclampsia. At the same time, the level of Hcy in HDP is useful to predict the progression of disease.