啤酒泡沫活性蛋白质中Z4蛋白质的单克隆抗体制备(一)

Isolation and Identification of protein Z4 in Beer Foam-Positive Protein(I)

目的研制啤酒泡沫活性蛋白质中Z4蛋白质的单克隆抗体制备(mAb),用于啤酒生产中泡沫活性蛋白质的准确高效测定。方法Z4蛋白质与弗氏佐剂乳化后免疫BALB/C小鼠,采用间接ELISA法筛选阳性克隆株,按照传统杂交瘤技术制备单克隆抗体。结果成功地获得两株Z4蛋白质的单克隆抗体,为其啤酒泡沫蛋白质中Z4蛋白质基础研究及其鉴定奠定了基础。

Objective： To prepare specific monoclonal antibodies against Foam-Positive Proteins Z4 with efficient and accurate determina tion of the proteins in Beer. Methods： BLAB/c mice was immunized with purified proteins emulsified with Freund＇s adjuvant, monoclonal antibodies against Foam-Positive Proteins Z4 were prepared by tradition hybridoma technique, screening by enzyme-linked immunosor bent assay （EL/SA）. Results： We obtained two specific and stable monoclonal antibodies against Foam-Positive Proteins Z4; it provides basic research and the identification laid the foundation for Foam-Positive Proteins Z4.