MAGE-1与IL-18共表达DNA疫苗在小鼠体内诱导特异性免疫应答能力的研究

Study of specific immune response on mice induced by a DNA vaccine co-expressing both MAGE-1 and interleukin-18

目的:观察已构建的pcIL-18-MAGE共表达质粒疫苗接种小鼠所引起的免疫应答情况。方法:质粒大量制备肌注小鼠,每隔10天加强免疫1次,共免疫3次。末次免疫后7天,收集血清及脾细胞,流式细胞术检测小鼠T细胞亚群情况及血清中抗MAGE-1多抗的产生,MTT法检测小鼠CTL杀伤活性。结果:pcIL-18、pcMAGE-1、pcIL-18+pcMAGE-1及pcIL-18-MAGE免疫组CD3+、CD4+、CD8+、CD4+/CD8+及MAGE-1抗体的产生均高于pcDNA3免疫组(P<0.05),且pcIL-18-MAGE共表达免疫组高于pcIL-18+pcMAGE-1共同注射组(P<0.05)。pcIL-18-MAGE免疫组对SMMC-7721的杀伤活性最高。结论:pcIL-18-MAGE共表达基因疫苗接种小鼠与其它实验小鼠相比,可以有效产生更好的特异性免疫应答。

Objective： To observate immunologic response of immunized mice by the vaccine pcIL- 18-MAGE. Methods：Abundant plasraids were extracted and immunized mice, two booster injection were carried out at ten days intervals. Seven days after the third immunization, serum antibody against MAGE-1 and T cell subgroup were detected by FCM, and CTL killing activity were assayed through MTT. Results：About serum antibody against MAGE-1 and T cell subgroup,recombinant plasmids groups were all higher than the vacant plasmid pcDNA3 group（P 〈 0.05）,and the coexpression group was higher than the group injected both pcIL-18 and pcMAGE-1 （P 〈 0.05）. pcIL-18-MAGE group＇s killing activity was highest against SMMC-7721. Conclusion：The coexpmssion vaccine pclL-18-MAGE can induce better specific immunological responses on mice compared with other experimental groups.