人参总皂苷和小檗碱对肺癌PG细胞“Fas反击”的影响

The effect of Ginsenoside and Berberine on "Fas counterattack" of lung carcinoma cell line PG

目的:探讨人参总皂苷(Ginsenoside,Gs)和小檗碱(Berberine,Ber)对肿瘤"Fas反击"免疫逃逸机制的影响。方法:首先建立肺癌PG细胞与Jurkat细胞的共培养体系;Giemsa染色和流式细胞仪观察和检测共培养后的Jurkat细胞的凋亡;SABC免疫细胞化学法检测Gs和Ber处理后的PG细胞FasL、Fas分子的表达。结果:Jurkat细胞与PG细胞共培养后产生凋亡,Gs和Ber处理的PG细胞诱导的Jurkat细胞凋亡更为显著;Gs和Ber可以上调PG细胞FasL、Fas分子的表达。结论:Gs和Ber可以促进PG细胞诱导Jurkat细胞凋亡的作用,其机制可能与Gs和Ber上调PG细胞FasL分子的表达有关。

Objective：To investigate the effect of Ginsenoside and Berberine on tumor ＂Fas counterattack ＂mechanism.Methods：The co-culture model was built with Jurkat plus PG cells firstly. Giemsa staining and flow cytometry were used to observe apoptosis of Jurkat after co- cultured with PG cells. The expressions of FasL and Fas molecules were evaluated by immunocytochemistry SABC method. Results： The Jurkat incurred apoptosis after co-cultured with PG cells. The apoptosis was more obvious when PG cells were treated with Ginsenoside and Berberine. Ginsenoside and Berberine could up-regulate the FasL and Fas expressions. Conclusion： Ginsenoside and Berberine can promote the apoptosis of Jurkat induced by PG cells. The up-regulation of FasL may play an important role in apoptosis induction.