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ING4基因对人胃癌细胞SGC-7901生物行为的影响 被引量：8

Effect of ING4 gene transfection on biological characteristics of human tumor cell line SGC-7901
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摘要 目的:研究人ING4对人胃癌细胞SGC-7901的影响,探讨其作用机制。方法:将重组腺病毒质粒pAd-ING4用PacI线性化,经脂质体转染QBI-293A细胞,获得重组病毒Ad-ING4。Ad-ING4感染人SGC-7901细胞,RT-PCR及Western blot分析ING4和p21表达,MTT法检测Ad-ING4对细胞生长的影响,激光共聚焦显微镜检测细胞凋亡。结果:Ad-ING4感染后,SGC-7901细胞中有ING4 mRNA和蛋白质表达,细胞生长受到明显抑制,与野生型SGC-7901细胞相比,p21表达水平增高,细胞凋亡率明显增高,P<0.05。结论:ING4可通过上调p21表达发挥抑制SGC-7901细胞生长、诱导细胞凋亡的作用。 Objective：To investigate the effect of IGN4 gene trasfection on SGC-7901 cells, and the possible mechanism for its anti-tumor effect .Methods： Ad-ING4 was obtained by gene recombination and packaging technique in vitro. The expression of ING4 mRNA and protein were analyzed by RT-PCR and western-blot respectively. The effect of Ad-ING4 on growth of SGC-7901 cells was detected by MTT. Apoptotic ceils were detected by Laser Scan Co-focal Microscope （LSCM）. The expression of p21 in SC, C-7901 cells was analyzed by western-blot, Results： After infection with Ad-ING4, the expression of ING4 mRNA and protein were showed in SGC-7901 cells detected. The proliferation of SGC-7901 cells was inhibited significantly（ P 〈 0.05）, acompanied by increased cell apoptosis. The expression of p21 was higher than in wildtype SGC-7901 cells. Condusion：Ad-ING4 can inhibit the growth of SGC-7901 cells by up-regulating the expression of p21.
作者 黄俊琼 孙万邦 张海峰 杨吉成
机构地区 遵义医学院珠海校区免疫学教研室 苏州大学医学院细胞与分子生物学教研室
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2008年第7期612-614,617,共4页 Chinese Journal of Immunology
关键词 ING4 AD-ING4 人SGC-7901细胞 P21 ING4 Ad-ING4 Human SGC-7901 cells P21
分类号 R73-3 [医药卫生—肿瘤]
  • 相关文献

参考文献12

  • 1Shiseki M, Nagashima M, Pedeux R M et al. P29ING4 and p28ING5 bind to p53 and p300, and enhance p53 activity[ J]. Cancer Research, 2003;63 (10) : 2373-2378.
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二级参考文献7

  • 1Igorl G, Sergey K, Olga C, etal. The canditae tumour suppressor protein ING4 regulates brain tumour growth and angiogenesis.Nature, 2004,428(6980) : 328-332.
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  • 6付浩,袁媛,孙秀菊,王兰,马明超,李福才,赵彦艳,王吉民,孙开来.IL-8基因在胃癌及癌前状态中的表达[J].中国肿瘤临床,2002,29(4):237-240. 被引量：15
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共引文献13

同被引文献64

  • 1解先宽,杨迪生,叶招明,陶惠民.反义c-myc重组腺病毒的构建及其抗骨肉瘤细胞的作用[J].癌症,2005,24(3):292-297. 被引量：24
  • 2万美珍,徐建忠,史永娟,陈玉芳,汪美华,潘耀东.进展期胃癌组织中COX-2表达与树突状细胞浸润的相关性研究[J].中华肿瘤防治杂志,2006,13(13):1005-1007. 被引量：8
  • 3章春花,张海峰,盛伟华,王金志,叶震敏,杨吉成,缪竞诚.mING基因的腺病毒载体的构建与表达[J].苏州大学学报（医学版）,2006,26(4):547-549. 被引量：4
  • 4Satoh T, Teh BS, Timme TE, et al. Enhanced systemic T- cell activation after in situ gene therapy with radiotherapy in prostate cancer patients [J]. Int J Radiat Oncol Biol Phys, 2004,59 (2) : 562-571.
  • 5Shiseki M, Nagashima M, Harris CC, et al. p29ING4 and p28ING5 bind to p53 and p300, and enhance p53 activity[J]. Cancer Res, 2003,63(10) :2373-2378.
  • 6Garkavtsev I, Kozin SV, Jain RK, et al. The candidate tumour suppressor protein ING4 regulates brain tumour growth and angiogenesis [ J ]. Nature, 2004,428 (6980) : 328-332.
  • 7Ozer A, Wu LC, Bruick RK. The candidate tumor suppressor ING4 represses activation of the hypoxla inducible factor (HIF) [J]. Proc Natl Acad Sci USA, 2005,102(21) :7481- 7486.
  • 8Gunduz M, Nagatsuka H, Nagai N, et al. Frequent deletion and down-regulation of ING4, a candidate tumor suppressor gene at 12p13, in head and neck squamous cell carcinomas [J]. Gene, 2005,356: 109-117.
  • 9Xie YF, Zhang HF, Yang JC, et al. Adenovirus-mediated ING4 expression suppresses lung carcinoma cell growth via induction of cell cycle alteration and apoptosis and inhibition of tumor invasion and angiogenesis[J]. Cancer Letters, 2008, 271(1): 105-116.
  • 10Xie YF, Sheng WH, Yang JC, et al. Adenovirus-mediated ING4 expression suppresses pancreatic carcinoma cell growth via induction of cell cycle alteration, apoptosis and inhibition of tumor angiogenesis [J]. Cancer Biother Radiopharm, 2009, 24(2) : 261-269.

引证文献8

二级引证文献27

中国免疫学杂志
2008年 第7期

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