早孕期人蜕膜趋化因子CCL2及其受体CCR2的表达及意义

Expression of chemokine CCL2 and its receptor CCR2 in decidua and decidual stromal cells of human first trimester gestation

目的:分析人早孕蜕膜及蜕膜基质细胞趋化因子受体CCR2及其配体CCL2在人早孕蜕膜组织及蜕膜基质细胞的表达和分泌,以探讨CCR2/CCL2在母-胎界面的生物学作用。方法:收集早孕期蜕膜组织,分离蜕膜基质细胞,分别用半定量RT-PCR、免疫化学方法分析正常人早孕蜕膜组织和培养的人蜕膜基质细胞CCR2/CCL2表达;并且用流式细胞术和ELISA法分别检测蜕膜基质细胞表面CCR2的表达和培养的蜕膜基质细胞上清中CCL2的分泌。结果:人早孕蜕膜组织和蜕膜基质细胞均高水平转录和翻译CCR2/CCL2,培养的基质细胞能分泌大量的CCL2,其分泌量呈时间依赖性。结论:早孕蜕膜高表达和分泌CCR2/CCL2可能参与早孕期母-胎免疫调节。

Objective：To investigate expression of the ehemokine CCL2 and its receptor CCR2 in human decidua and decidual stromal cells （DSC） of the first trimester gestation. Methods：The deciduae were from women who had undergone an artificial abortion at 5-11 weeks of normal gestation. The total RNA was extracted by using the TRIzol reagent,from decidua or the Percoll-gradient-isolated DSC. The expressions of CCR2 and CCL2 in deciduas and DSC were determined by way of semi-quantitative reverse transcriptase polymerase chain reaction （RTPCR）, immtmocytochemistry, immtmohistochemistry, flow eytometry and ELISA, respectively. Results： CCR2 and CCL2 were highly expressed in decidua and DSC. The DSC could secret CCL2 in a time-dependent manner, and the highest concentration was beyond 20 ng/ml. Conclusion： CCR2 and CCL2 have been expressed in decidua and DSC of human first trimester gestation, and may play an important role in the decidual immune cell constitution in human early gestation.