红细胞补体受体1水平在系统性红斑狼疮发病作用的研究

Levels of erythrocyte complement receptor type 1 in the pathogenesis of systemic lupus erythematosus

目的:研究红细胞补体受体1(Erythrocyte receptor type 1,E-CR1)水平与系统性红斑狼疮(Systemic lupus erythematosus,SLE)疾病活动性及免疫异常之间的相关性,探讨E-CR1在SLE发病中的作用。方法:使用流式细胞仪检测技术测量了72例SLE患者和20例正常对照者末梢血中E-CR1水平,同时测定SLE患者血清补体(C3、C4)、免疫球蛋白(IgG、IgA、IgM)、γ-球蛋白(γ-G)及血沉,测定SLE患者血细胞计数,包括:白细胞(WBC)、红细胞(RBC)、血红蛋白(HGB)、红细胞比容(HCT)、平均红细胞容积(MCV)及血小板(PLT)。对72例SLE患者做狼疮活动性测量(SLAM)评分。结果:SLE患者E-CR1水平明显低于对照组(20.25±9.15/57.00±10.41,P<0.01)。在SLE患者中,E-CR1水平与IgG、γ-G及血沉呈明显的负相关(P<0.05);与HGB有非常明显的正相关(P<0.01),与HCT有明显正相关性(P<0.05);与SLAM呈显著线性负相关(P<0.01)。活动期SLE患者(n=46)E-CR1水平明显低于非活动期SLE患者(n=26)E-CR1水平(P<0.05)。在活动期SLE患者中,E-CR1水平与RBC、HGB、HCT均有非常明显正相关性(P<0.01);与血沉和SLAM有负的线性相关(P<0.011)。结论:E-CR1水平降低可能是SLE发病的起始环节之一;免疫球蛋白(IgG及γ-G)水平升高及RBC、HGB、HCT降低可能与E-CR1水平进一步降低有关,从而使SLE病情加重;E-CR1可作为SLE疾病活动性指标之一。

Objective： To investigate the correlationship between levels of erythrocyte receptor typel （E-CR1） and disease activity and abnormal criteria of immunology in systemic lupus erythematosus （SLE）and to study the E-CR1 levels in pathogenesis of SLE. Methods： The fluorescence intensity in sere from 72 patients with SLE and 20 cases of normal controls was detected by flucytometry. Complement（C3, C4） ,im- munoglobulins（IgG,IgA and IgM）,gammaglobulins（γ-G）, erythrocyte sediment rate（ESR） and blood cell counts, including white blood cell （WBC）, red blood cell（RBC）, hemoglobin（HGB）, hematocrit（HCT）, mean corpuscular volume（MCV） and platelet（PLT） were detected at the same time.The scoring instrmnents of systemic lupus activity measure （SIAM） were used to evaluate disease activity.Results：The E-CR1 levels in patients with SLE were lower significantly than that in normal controls （P 〈 0.01 ）. In the 72 patients with SLE, E-CR1 levels were showed in a linear negative-correlation with IgG, γ- G and ESR（ P 〈 0.05）, while in a linear positive-correlation significantly with HGB（ P 〈 0.01 ）, and with HCT（ P 〈 0.05）, and linear negative-correlation significantly with SIAM（ P 〈 0.01 ）. E-CR1 levels in active SLE （ n = 46） were lower than that in inactive SLE （n = 26） （P 〈 0.05）. In active SLE, E-CR1 levels were showed linear positive-correlation significantly with RBC, HGB and HCT（ P 〈 0.01 ）, and a linear negativecorrelation with ESR and SLAM （ P 〈 0.05）. Conclusion： The decreased levels of E-CR1 might be one of causes in pathogenesis of SLE. Higher levels of lgG and γ- G and decreased RBC, HGB and HCT consequently result in decreasing E-CR1 and deterioration of SLE. E-CR1 levels could be used as one of the monitors for SLE disease activity.