烟叶中26种挥发性与半挥发性有机酸的GC-MS/SIM法同时分析

Simultaneous Determination of 26 Volatile and Semi-volatile Organic Acids in Tobacco by GC-MS/SIM

采用加速溶剂萃取,以二氯甲烷-乙腈（体积比1∶2）为萃取溶剂,N,O-双（三甲基硅基）三氟乙酰胺硅烷化,反-2-己烯酸和肉桂酸为内标,GC-MS/SIM法同时测定了烟叶中的甲酸、乙酸、乳酸、苯甲酸、十六酸、十八酸等26种挥发性、半挥发性有机酸。方法的回收率为81%-106%,相对标准偏差为1.73%-7.08%,检出限为0.1-13.72μg/g。以该方法对部分烟叶样品中挥发性、半挥发性有机酸进行了定量分析。

The volatile and semi-volatile organic acids in cigarettes were extracted with a dichloromethane-acetonitrile（1 ∶2,by volume） mixture solvent by accelerated solvent extraction（ASE）,then derivatized with NO-bis（trimethylsilyl）trifluoroacetamide.The GC-MS/SIM method for simultaneous determination of 26 organic acids,including formic acid,acetic acid,lactic acid,benzoic acid,hexadecanoic acid,octadecanoic acid etc.,was developped by using trans-2-hexenoic acid and cinnamic acid as dual internal standards.The recoveries of the method ranged from 81%to 106%with RSDs of 1.73%-7.08%.The limits of detection ranged from 0.1 to 13.72 μg/g.The contents of volatile and semi-volatile organic acids in some tobacco samples were determined using this method.