桃红四物汤水提液中没食子酸、芍药苷和红花黄色素含量的测定

Determination of gallic acid,paeoniflorin and safflor yellow in Taohong Siwu Decoction

目的：测定桃红四物汤水提液（桃仁、红花等）中没食子酸、芍药苷和红花黄色素的含量。方法：没食子酸、芍药苷和红花黄色素含量分析采用高效液相色谱法。没食子酸色谱条件：流动相：甲醇-磷酸水溶液（pH=3.0）（5∶95）,柱温35℃,检测波长：271 nm。红花黄色素色谱条件：流动相：甲醇-磷酸水溶液（pH=3.0）（80∶20）,柱温25℃,检测波长：403nm。芍药苷色谱条件：流动相：甲醇-磷酸二氢钾溶液（38∶62）,检测波长：230 nm,柱温：25°C。流速均为1.0 mL/min,进样量均为：20μL。结果：HPLC测定没食子酸在4.547×10^-3～1.455×10^-1μg/μL（r=0.9993）范围内线性关系良好,平均回收率为98.0%,RSD为1.8%。芍药苷在0.010～0.200μg/μL（r=0.999 8）范围内线性关系良好,平均回收率为98.4%,RSD为0.69%。红花黄色素在2.34×10^-2～7.50×10^-1μg/μL（r=0.9994）范围内线性关系良好,平均回收率为99.2%,RSD为0.88%。结论：本法简单快捷,重现性好,可作为桃红四物汤中没食子酸、芍药苷和红花黄色素的质控方法。

AIM： To determine gallic acid, paeoniflorin and safflor yellow in Taohong Siwu Decoction （Semen Persicae,Flos Carthamietc. ） METHODS： To analyse three constituents by HPLC on a Hypersil BDS C18 column and gallic acid detection wavelength was set at 271 nm. The mobile phase was methanol-water （5：95 ） （adjusted to pH =3.0 with H3PO4） at a flow rate of 1.0 mL/min. Safflor yellow detection wavelength was set at 403 rim. The mobile phase was methanol-water （80： 20） （ adjusted to pH = 3.0 with H3PO4 ） at a flow rate of 1.0 mL/min. Paeoniflorin detection wavelength was set at 230 rim. The mobile phase was methanol-KH2PO4 at a flow rate of 1.0 mL/ min. RESULTS： Gallic acid showed a good linear relationship at the range of 4. 547 × 10^-3 - 1. 455 × 10^-1 μg/ μL （r = 0. 999 3 ）. Paeoniflorin showed a good linear relationship at the range of 0. 010-0. 200 μg/μL （ r = 0. 999 8 ） and saffior yellow showed a good linear relationship at the range of 2.34 × 10^ -2 - 7.50 × 10^ -1 μg/μL （ r =0. 999 4）. The average recoveries were 98.0% for gallic acid,98.4% for paeoniflorin and 99.2% for safflor yellow. The RSD of the method were 1.8% ,0.69% and 0.88%, respectively. CONCLUSION： The method is proved to be fast, feasible, simple, and can be used for quality control of gallic acid, paeoniflorin and safflor yellow in Taohong Siwu Decoction.