苦参素抑制人膀胱癌T24细胞生长作用研究

Effects of Oxymatrine on Human Bladder Cancer T24 Cell Line

目的探讨苦参素对人膀胱癌T24细胞的抑制作用及其可能机制。方法MTT法检测T24细胞的生长抑制率;流式细胞仪检测细胞周期改变和免疫组化,SP法检测Bcl-2和Bax蛋白表达的变化。结果2 mg.mL-1苦参素作用24和72 h后细胞生长抑制率为14.10%和49.67%;8 mg.mL-1苦参素作用24和72 h后细胞生长抑制率为35.40%和80.50%。随着苦参素浓度的增加,G0/G1期T24细胞所占比例逐渐上升,G2/M期细胞所占比例逐渐下降;且细胞内Bcl-2表达下调,Bax表达上调。结论苦参素通过诱导癌细胞周期阻滞于G0/G1期来抑制人膀胱癌T24细胞的生长增殖。其机制可能与下调Bcl-2和上调Bax蛋白表达,诱导癌细胞凋亡有关。

Objective To study antitumor effects and mechanism of oxymatrine （OMT） on human bladder cancer T24 cell line in vitro. Methods Cell suppression rates were detected by MTT assay, cell cycle was measured by flow cytometry and expressions of bcl-2 and bax protein were detected by immunohistochemistry. Results Cell suppression rates were 14.10% and 49.67%, after treated with 2 mg · mL^-1 OMT ibr 24 and 72 h, respectively. The rates increased to 35.40% and 80.50% by 8 mg · mL^-1 OMT. The ratio of G0/G1 phase cells was increased and the ratio of G2/M phase cells was decreased. Bcl-2 protein expression was down-regulated and Bax protein expression was up-regulated after different treatment of OMT. Conclusion OMT could inhibit the growth of human bladder cancer 324 cell by inducing cell cycle arrest at G0/G1 phase, which may be related to the down-regulation of Bcl-2 protein expression and up-regulation of Bax protein expression.