Survivin-siRNA在体外诱导Panc-1细胞凋亡的实验研究

The Study of siRNA of Survivin Gene Induced Apoptosis in Panc1 Cell Line in Vitro

目的:探讨RNA干扰技术抑制Survivin对人胰癌细胞株Panc-1细胞凋亡的影响。方法:体外设计、合成针对sur-vivin基因的小分子干扰RNA(survivin-siRNA),应用脂质体介导survivin-siRNA转染Panc-1细胞后,MTT试验测定转染细胞的增殖抑制率,RT-PCR检测细胞survivin mRNA表达,琼脂糖凝胶电泳分析其对Panc-1细胞凋亡诱导作用。结果:转染survivin-siR-NA的Panc-1细胞增殖明显受抑制,与对照进行比较,具有显著性差异(P<0.01);经琼脂糖凝胶电泳,转染survivin-siRNA的Panc-1细胞可见到DNA梯形条带,而对照细胞未见到。结论:survivin-siRNA能够显著诱导Panc-1细胞凋亡。

Objective： To explore the effects of surviving-siRNA on induced apoptosis in human pancreatic cancer cell line Panc-1. Methods. The small interference RNA （siRNA） targeting survivin gene was designed and synthesized by transcription in vitro. Survivin siRNA was transferred into Panc-1 cell line by lipofection. The opposite livability on Panc-1 cell line was assayed with MTT test. Expression of survivin was detected by RT-PCR. Apoptosis was detected by DNA gel electrophoresis. Results： Survivin siRNA obviously inhibited the Panc-1 cells growth （P〈0.01）. Agarose gel electrophoresis of genomic DNA from Survivin siRNA transferred Pane-1 cells showed typical DNA ladder, but DNA from other cells did not. Conclusion： Survivin siRNA could effectively induce apoptosis in Pane-1 cells.