摘要
目的研究钙蛋白酶10基因Indel-19与2型糖尿病及胰岛素抵抗的关系。方法选取120例2型糖尿病患者和92例非糖尿病健康对照者,用聚合酶链反应法(PCR)扩增目的基因,琼脂糖电泳,检测所有受试者的Indel-19基因型,采用生化技术测定空腹血糖、血脂以及空腹胰岛素水平(FINS),用胰岛素抵抗指数HOMA-IR评价胰岛素抵抗。结果2型糖尿病组较对照组有较高的TG、TC、LDL、BMI和IR水平(P<0.05),两组钙蛋白酶10基因Indel-19基因型22的频率分别为54.2%和44.6%,差异无统计学意义;在2型糖尿病人群中Indel-19基因型22的个体较基因型11和12的个体有较高的FINS、IR水平和较低的高密度脂蛋白(HDL)水平(P<0.01,P<0.05)。结论钙蛋白酶10基因Indel-19的基因型22与江苏地区2型糖尿病人群的胰岛素抵抗相关。
Objective To study the correlations between calpain 10 gene polymorphism and insulin resistance in type 2 diabetes.Methods 120 type 2 diabetes patients and 92 normal control subjects were investigated;polymerase chain reaction（PCR） was used to determine the genotypes of site Indel-19 in calpain 10 gene.Results Higher TG、TC、LDL、BMI and IR were found in type 2 diabetes patients（P〈0.05）;No difference was found in the ditribution of genotype of site Indel-19 between the two groups;In the type 2 diabetes group,higher FINS、IR and lower HDL were found in the individuals with genotype 22 than those of individuals with genotype 11 and 12（P〈0.01,P〈0.05）.Conclusion The study suggested that Indel-19 of Calpain 10 gene correlated to insulin resistance in type 2 diabetes.
出处
《四川医学》
CAS
2008年第7期832-834,共3页
Sichuan Medical Journal
基金
镇江市科技计划项目(SH20050049)
