扶正解毒汤对硫酸镍遗传毒性拮抗作用

Effects of Fuzhengjiedu decoction on heritable toxicity induced by nickel sulfate

目的研究灌服复方中药扶正解毒汤（FJD）后兔血清对硫酸镍（NiSO4）遗传毒性的抑制作用。方法采用^3H—TdR掺入法，观察扶正解毒汤含药血清对NiSO4诱导的体外培养人支气管上皮细胞（16HBE）非程序DNA合成（UDS）的影响。结果NiSO4（100～200umol／L）暴露组细胞UDS水平明显高于阴性对照组（P〈0．05，P〈0．01）；高、中、低剂量扶正解毒汤血清对16HBE细胞UDS均无诱导效应，但与NiSO4（200umol／L）共同处理后细胞的UDS水平[CPM分别为（221．45±34．93），（289．51±48．26），（672．47±56．68）]；均低于NiSO4暴露组[CPM为（998．63±81．36），P〈0．05，P〈0．01]，FJD血清对NiSO4诱导的UDS有显著的抑制作用，且呈剂量-效应关系（r=0．837，P〈0．05）。结论体外实验结果显示，扶正解毒汤本身无致突变性，具有拮抗硫酸镍遗传毒性的作用及抗镍致癌潜能。

Objective To study the effects of Fuzhengjiedu decoction（FJD） on the genotoxicity induced by nickel sulfate （NiSO4）. Methods By using 16HBE cell in vitro with 3H - TdR incorporation assay techniques, the effects of FJD on nickel induced unscheduled DNA synthesis（UDS） were observed. Results The UDS induced by NiSO4 （ 100 - 200 umol/L） in 16HBE cells increased significantly compared with negative control（ P 〈 0.05, P〈 0.01 ）. FJD could not induce UDS. In cells co - treated with various doses of FJD and NISO4（200 umol/L） the UDS levels were lower than that of induced by NiSO4 （CPM = 998.63 ± 81.36） （ P 〈 0,05, P 〈 0. 01 ）. FJD could inhibit the UDS induced by NiSO4 in dose - dependent manner （r=0.837, P 〈0.05）, Conclusion FJD were not mutagenic and could have inhibitory effects on the genotoxicity and carcinogenicity of nickel.