摘要
目的:建立以反相高效液相色谱法测定3种不同药物剂型样品中菊苣酸含量的方法。方法:色谱柱为VP-ODSC18,流动相为甲醇-1%醋酸-四氢呋喃(33:62:5),流速为1.0mL.min-1,检测波长为327nm,柱温为30℃,进样量为20μL。结果:菊苣酸进样量的线性范围为0.39872~3.9872μg(r=0.9998);口服液、胶囊剂及片剂的平均回收率分别为99.85%(RSD=0.98%)、102.50%(RSD=1.84%)、100.50%(RSD=1.69%)。结论:本方法准确度高、重现性好、专属性强,可用于菊苣酸的含量测定。
OBJECTIVE: To determine the contents of chicoric acid in three different pharmaceutical dosage forms by RP - HPLC. METHODS: Samples were determined on VP - ODS C18, with the mobile phase consisted of methanol 1% acetic acid tetrahydrofuran (33 : 62 : 5) with flow rate at 1.0 mL · min^-1, UV detection wavelength at 327 nm, column temperature at 30 ℃ and sample size at 20 μL. RESULTS: The linear range of chicoric acid was 0.398 72--3.987 2 μg(r = 0.999 8). The average recoveries of the oral liquid, capsules, and tablets were 99.85 % (RSD = 0. 98 % ), 102.50 % (RSD=1.84 % ), and 100.50% (RSD = 1.69% ), respectively. CONCLUSION: This method is accurate, reproducible, specific, and suitable for the content determination of chicoric acid.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第22期1739-1741,共3页
China Pharmacy