停跳液细菌内毒素检查

Tachpleus Amebocyte Lysate Test for Bacterial Endotoxins in Cardioplegia Solution

目的:建立停跳液的细菌内毒素检查方法。方法:根据2005年版《中国药典》(二部)附录细菌内毒素检查法进行试验。结果:停跳液稀释至8倍时对细菌内毒素的检查无干扰,Et在0.5Es～2Es范围内。结论:停跳液用灵敏度为0.06EU.mL-1的鲎试剂检查细菌内毒素是可行的。

OBJECTIVE： To establish the method of assaying bacterial endotoxin in cardioplegia solution. METHODS： The tachpleus amebocyte lysate （TAL） test for bacterial endotoxin in cardioplegia solution was performed in accordance with Chinese Pharmacopoeia （2005 Edition, Volume Two） . RESULTS： The 8-fold diluents of cardioplegia solution did not interfere with the test for bacterial endotoxins, with Et = 0.5 Es-- 2 Es. CONCLUSION ： It is feasible to detect bacterial endotoxins in cardioplegia solution with TAL at a sensitivity of 0.06 EU· mL^-1.