转录间隔区(ITS)在寄生虫分子生物学分类中的应用及其进展

ADVANCES AND APPLICATION OF RDNA-ITS IN THE MOLECULAR TAXONOMY OF PARASITE

在寄生虫分子分类学研究中,利用核糖体DNA对寄生虫进行分类学,以及虫种进化关系学的研究,已经成为国际上普遍应用的方法。但由于它具有高度保守性的特点,所以很难在同种异株的区分上有所作为。内转录间隔区ITS是位于寄生虫的遗传物质特别是核糖体DNA(rDNA)上18S和28S基因之间的区域片段,由于它在生物种和亚种间变异较大,从而可以用来进行动物原虫种间以及株间的分类鉴定。因此,在分子分类学领域,越来越多的采用核rDNA的内转录间隔区(ITS)作为分子标记。本文介绍ITS作为分子标记在寄生虫分子分类中的作用及其进展。

It is a general method that using ribosome RNA gene to identify the parasites and study the species evolution relation in molecular taxonomy, However, rRNA is very conservative there is little change among strain within one species, so it is very difficult to identify different strains of homogeneity. ITS rDNA, locating between 18S and 28S, has very high variation in biological species, was and can be used to distinguish of animal protozoon species. Recently, many papers on molecular taxonomy of parasites were published using ITS as a molecule marker. The paper described the role and progression of the ITS as a molecule mark in parasite molecule classification.