HPLC法测定益母草中盐酸水苏碱的含量

Determination of stachydrine hydrochloride in leonurus japonicus houttby HPLC

目的建立益母草中盐酸水苏碱含量的HPLC测定方法。方法采用磺酸基团键合硅胶的阳离子交换剂（SCX）为填充剂的色谱柱；流动相为乙腈-0．05mol·L^-1磷酸二氢钾-磷酸（15：85：0．15）；检测波长为192nm。结果盐酸水苏碱在进样量为0．14～11．18μg范围内具有良好的线性关系，r=0．9999；盐酸水苏碱的平均回收率为98．95％，RSD为1．21％（n=6）。结论本方法专属性强，重现性好，可作为益母草中盐酸水苏碱的定量测定方法。

Aim To establish a method for determination of stachydrine hydrochloride in leonurus japonicus houtt by HPLC . Methods The chromatographic column was shim-park Hypersil SCX column （250 mm× 4.6ram,5.0 μm）. The mobile phase was acetonitrile-potas- sium dihydrogen phosphate-phosphoric acid（ 15： 85： 0.15） and UV detection was performed at 192 nm. Results The calibration curve was linear over the range between 0. 14 and 11.18μg of stachydrine（r =0. 9999）. The average recover was 98.95% and RSD was 1. 21% （n = 6）. Conclusion This method is selective and reproducible, and can be used to determine stachydrine hydrochloride in leonurus japonicus houtt.