徐长卿中丹皮酚含量测定方法研究

Determination of paeonol in radix etrhizoma cynanchi paniculati

目的建立HPLC法测定徐长卿中丹皮酚的含量。方法采用C18色谱柱，甲醇-水（45：55）为流动相，柱温35℃，流速1．0ml·min^-1，检测波长为274nm。结果丹皮酚在0．0352～0．2816μg范围内与峰面积值呈现良好的线性关系；r=0．9999（n=5），平均回收率=100．96％（RSD=1．9％，n=5）。结论本法操作简便、准确可靠，可用于徐长卿的质量控制。

Aim To determine the content of paeonol in radix etrhizoma cynanchi paniculati by HPLC. Method A C18 column was used,methanol-water （45 at 55） as mobile phase with UV detection at 274 nm,flow rate of 1.0 ml · min^-1 ,and column temperature： 35℃. Results The calibration curve was in good linearity over the range between 0.035 2 and 0. 281 6μg, r = 0. 999 9 （ n = 5 ） . The average recovery was 100. 96% （n = 5） and RSD was 1.9%. Conclusion The method was simple, reliable, accurate and can be used for the quality control of radix etrhizoma cynanchi paniculati.