壁虎鲜品和炮制品抗肿瘤活性比较研究

Comparative Research on Anticancer Activity between Fresh and Processed Gekko subpalmatus

目的:比较中药壁虎鲜品与炮制品对小鼠H22肝癌细胞体内外生长抑制作用的强弱。方法:体外试验采用MTT法,检测壁虎提取液冻干粉对H22细胞的增殖抑制情况;体内试验采用小鼠肝癌H22移植瘤法,比较对实体肿瘤的抑制作用,并检测药物对小鼠胸腺指数及脾指数的影响。结果:在体外试验中,壁虎鲜品和炮制品对H22细胞的半数抑制率IC50分别为8.53、6.42 mg/ml;体内试验,鲜品和炮制品最大抑瘤率分别为53.0%和47.4%。炮制品各组的胸腺指数和脾指数均高于环磷酰胺(CTX)阳性对照组,鲜壁虎的中、高剂量组胸腺指数和低剂量组脾指数较阳性对照组高。结论:鲜品与炮制品均有抗肿瘤作用,但二者活性差异不显著;炮制品对胸腺和脾脏有生长刺激作用。

Objective：To investigate the anticancer activity of fresh and processed Gekko subpalmatus on mouse H22 hepatocellular in vitro and in vivo comparatively.Methods：In vitro,the effect of different concentration fresh and processed Gekko subpalmatus on proliferation of H22 cells were measured by Monotetrazolium（MTT） assay. Mouse transplanted hepatoma H22 model was established to evaluate the anti-tumor activity of fresh and processed Gekko subpalmatus.Rate of restraining tumor,index of thymus and spleen were calculated after 14 days′ treatment.Results：In vitro,fresh and processed Gekko subpalmatus inhibited proliferation of H22 cells and the IC50 values were 8.53 mg/ml and 6.42 mg/ml.In vivo,the restraining tumor rates of fresh and processed Gekko subpalmatus each at the highest doses were 53.0% and 47.4%.Compared with those in CTX group,the indexes of thymus and spleen were markedly increased after all processed group′s treatment.The indexes of the thymus increased after low dose fresh group′s treatment.The indexes of the spleen of the moderate and high dose groups were higher than CTX group.Conclusion：Fresh and processed Gekko subpalmatus show significant anti-tumor activity in hepatoma cells both in vitro and in vivo.The processed samples can improve the immunity of the mouse.