复方丹参方与脂质体模拟生物膜的相互作用

Interactions of Fufang Danshen Prescription with Liposome Biomembrane

目的:研究复方丹参方中与脂质体有相互作用的潜在活性成分。方法:将脂质体作为模拟生物膜,采用平衡透析与高效液相色谱、质谱联用技术筛选和鉴定了复方丹参方中与脂质体有相互作用的成分,并考察了缓冲液pH值对复方丹参方与脂质体相互作用的影响。结果:复方丹参方中共有24个成分与脂质体相互作用明显,通过标准品对照及LC/TOF/MS方法鉴定了其中18个成分,包括7个丹参酚酸类成分、6个丹参二萜醌类成分和5个三七皂苷类成分。7个酚酸类成分分别是丹参素、原儿茶醛、丹酚酸E、迷迭香酸、紫草酸、丹酚酸B、丹酚酸A;6个二萜醌类成分分别是二氢丹参酮Ⅰ、四氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ、亚甲基丹参醌、丹参酮ⅡA;5个皂苷类成分分别是三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1、人参皂苷Rd、三七皂苷K。结论:脂质体平衡透析与高效液相色谱-质谱联用法,可有效地预测复方丹参方中多种成分在体内的吸收情况,揭示该复方的潜在活性成分,为其药效物质基础的进一步研究提供依据。

AIM： To study the interactions between Fufang Danshen Prescription （FDP） and liposome biomembrane. METHODS： Based on liposome equilibrium dialysis system, the constituents having interaction with liposome （as the model of biomembrane） in FDP were analyzed and identified by high performance liquid chromatography （HPLC） and time-of-flight mass spectrometry （TOF-MS） analysis. And the effect of pH value was also investigated on their interactions. RESULTS： Twenty-four constitutes in FDP interact with liposome obviously. Among them, eighteen compounds were identified in comparison with the standards in terms of UV spectrum, MS data along with retention time, including seven phenolic acids （danshensu, protocatechuic aldehyde, salvianolic acid E, rosmarinic acid, lithospermic acid, salvianolic acid B and salvianolic acid A）, six diterpenoid quinines （dihydrotanshinone Ⅰ, tetrahydrotanshinone Ⅰ, cryptotanshinone, tanshinone Ⅰ, methylene tanshiqunone and tanshinone IIA） and five saponins （notoginsenoside Rl, ginsenoside Rgl, ginsenoside Rbl, ginsenoside Ro and notoginsenoside K）. CONCLUSION： Liposome equilibrium dialysis system coupled with HPLC and LC/TOF/MS could simply and effectively estimate the absorption of multiple components in FDP in vivo, and predicts the potential bioactivity of these components, and thus contributes to the investigation of bioactive compounds with curative effect in FDP.