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酶标仪微板速率法检测ALT参数的优化 被引量:1

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摘要 目的:探讨酶标仪微板法检测ALT参数的选择。方法:采用动力学。结果:ALT的标本量为50μl,基质液为200μl;基质液温度为33C,酶标仪温度为30C;延迟时间为120s,测定时间120-360s;校正因数为1500;初始吸光度值下限值为0.686;板内和板问变异系数为1.85%、3.37%CPT得分100%。结论:该方法实现ALT自动化检测。
出处 《中国误诊学杂志》 CAS 2008年第24期5871-5872,共2页 Chinese Journal of Misdiagnostics
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