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Vitek-32微生物分析仪检测耐甲氧西林葡萄球菌评价

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摘要 目的以聚合酶链反应(PCR)检测mecA基因作为标准方法,评价Vitek-32微生物分析仪检测耐甲氧西林金黄色葡萄球菌(MRSA)及凝固酶阴性葡萄球菌(MRCNS)的可靠性。方法收集金黄色葡萄球菌72株,MRCNS64株,采用微生物分析仪Vitek-32配套的GPS-110卡检测MRSA与MRCNS,并与PCR及头孢西丁纸片扩散法进行比较。结果Vitek-32微生物分析仪检测MRSA及MRCNS的灵敏度及特异性分别为100.0%和89.7%、100.0%和80.0%;头孢西丁纸片扩散法的灵敏度及特异性均为100.0%。结论微生物分析仪Vitek-32操作简单、快速,检测MRSA及MRCNS的灵敏度高,是一种较好的方法。对于可疑株,用头孢西丁纸片扩散法复查可提高特异性。
作者 罗湘蓉 陈燕
出处 《检验医学与临床》 CAS 2008年第16期995-996,共2页 Laboratory Medicine and Clinic
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参考文献3

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