三种真空采血管对部分血清酶检测结果的影响分析

Research on some sero-enzymes measurement by applying three different vacuum tubes for venous blood samples

目的探讨无抗凝管、促凝管、分离胶促凝管制备的血清对丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、肌酸激酶(CK)、乳酸脱氢酶(LDH)等血清酶测定结果是否具有可比性,为临床检验提供实验数据。方法随机选取体检献血人员26例,空腹12h抽取静脉血,分别置于无抗凝管、分离胶促凝管、促凝管制备的血清在OLYMPUSAU5400生化分析仪上检测ALT、AST、LDH、CK4个项目。结果分别用无抗凝管与促凝管、分离胶促凝管制备的血清测定ALT、AST、LDH、CK差异无统计学意义,以无抗凝管作为标准管,临床可接受性能评价促凝管与分离胶促凝管均未超过临床接受误差范围。结论临床上可以采用分离胶促凝管和促凝管来代替无抗凝管制备血清,测定ALT、AST、LDH、CK项目。

Objective To discuss the comparability of ALT, AST, CK and LDH results by applying three different vacuum tubes （no additive tubes, separation gel coagulation promoting tubes and other coagulation promoting tubes） for venous blood samples, and to provide evidence for clinical tests. Methods Fast venous blood from 26 healthy blood donors were collected in test tubes without additives, separation gel coagulation promoting tubes and other coagulation promoting tubes. Olympus AU5400 Biochemical Analyzer was applied to examining serum ALT, AST, CK and LDH for all pre- pared serum samples. Results There was no statistical difference of serum ALT, AST, CK and LDH level collected in test tubes without additives, separation gel coagulation promoting tubes and other co- agulation promoting tubes. With the no additive tubes as the standard tubes, the above restult bias between separation gel coagulation promoting tubes and other coagulation promoting tubes were clinically acceptable. Conclusion There is credible comparability of serum ALT, AST, CK and LDH level among different kinds of venous blood samples collected in test tubes without additives, separation gel coagulation promoting tubes and other coagulation promoting tubes. The separation gel coagulation promoting tubes and other coagulation promoting tubes can substitute test tubes without additives for ALT, AST, CK and LDH determination.