薄层扫描法测定阴可净泡腾栓中苦参碱的含量

Determination of Matrine in Yinkejing Effervescent Suppository by TCL Scanning

用苯－醋酸乙酯－丙酮－浓氨试液（３∶３∶１∶０．１）为展开剂，以０．５％ＣＭＣ－Ｎａ硅胶Ｇ为吸附剂，薄层扫描法测定阴可净泡腾栓中苦参碱含量。样品测定波长为５２５ｎｍ，参比波长为６５０ｎｍ，苦参碱在１～５μｇ范围内呈线性关系。回归方程ｙ＝１３８４０．２８ｘ－５４２２．６７，ｒ＝０．９９８３，平均回收率为９６．８１％，ＲＳＤ为１．６７％。

Matrine of Yinkejing effervescent suppository was separated on 0.5%CMC_Na silica G plate with benzene_ethyl acetate_acetone_ammonia water (3∶3∶1∶0.1)as mobile phase. The sample was analyzed by CS_930 dual wave length TCL scanner at λs=525nm and λR=650nm. Linear relationship was recealed between 1 and 5μg(r=0.9983). The average recovery was 96.81%(n=5) and the RSD was 1.67%.