蛋白酶体抑制剂PS-341对白血病细胞株SHI-1的生长抑制作用研究

Inhibiting effect of proteasome inhibitor PS-341 on leukemic SHI-1 cells in vitro

目的探讨蛋白酶体抑制剂PS-341对膜连蛋白急性单核细胞白血病细胞株SHI-1的抑制作用。方法台盼蓝拒染法观察不同浓度PS-341对SHI-1细胞活力的影响,用异硫氰酸荧光黄(Annexin-V-FITC)、碘化丙啶(PI)检测60nmol/L浓度PS-341处理0、6、12、24h时的SHI-1细胞凋亡率,并用流式细胞仪检测细胞周期。结果PS-341呈时间和剂量依赖性抑制SHI-1细胞活力;60nmol/L浓度PS-341在0、6、12、24h诱导SHI-1细胞凋亡率分别为4.2%、8.5%、13.7%和30.1%;流式细胞仪检测显示,60nmol/L浓度PS-341处理SHI-1细胞0、6、12、24h,G1期的细胞比例分别为46.0%、30.0%、14.3%和0.8%,G2/M期的细胞比例分别为16.0%、25.5%、49.4%和69.0%。结论PS-341可以抑制SHI-1细胞生长,并能诱导其凋亡,使SHI-1细胞停留在G2/M期。

Objective To investigate inhibiting effect of proteasome inhibitor PS-341 on acute mononuclear leukemia SHI-1 cells. Methods Cultured SHI-1 cells were treated with 60 nmol/L of PS-341 for 0, 6, 12 and 24 h. Trypan blue exclusion assays was used to detect the cellular viabilities, FACS with Annexin-V staining was used to analyze cell apoptosis and cell cycle. Results PS-341 inhibited SHI-1 cells in a time- and dose-dependently manner. After PS-341 treatment for 0, 6, 12 and 24 h, apoptotic rates of SNI-1 cells were 4.2%, 8.5%, 13.7% and 30.1% respectively; FACS showed that G1 cells were 46.0%, 30.0%, 14.3% and 0.8% and G2/M cells were 16.0%, 25.5%, 49.4% and 69.0% respectively. Conclusion PS-341 can inhibit the proliferation of SHI-1 cells, induce apoptosis and block SHI-1 cells in G2/M stage.