镧对鲤鱼肾和鳃抗氧化性和酯酶同工酶的影响

Effects of lanthanum on antioxidant activity and esterase isozymes in kidney and gill of Cyprinus carpio Linnaeus

在实验条件下采用静水换水法,研究了不同浓度(0.01、0.1、2.0和5.0mg·L-1)的LaCl3对鲤鱼肾和鳃抗氧化性和酯酶(EST)同工酶的影响。结果表明:在LaCl3实验浓度范围内,处理4个月后,鳃中超氧化物歧化酶(SOD)活性受到显著抑制,肾中SOD活性、鳃中过氧化脂质(LPO)含量及0.01、0.1、5.0mg·L-1组肾中LPO含量与相应对照组相比均无显著性差异(P>0.05),2.0mg·L-1组肾中LPO含量却显著高于对照;0.01、0.1mg·L-1组,肾和鳃中过氧化物酶(POD)活性值均显著高于相应对照组(P<0.05或P<0.01),5mg·L-1组,肾和鳃POD活性均受到显著抑制;同工酶电泳实验结果表明:La3+能导致鲤鱼肾和鳃SOD、EST谱带亮度发生明显变化,鳃和肾EST同工酶原有的一些酶带消失,肾EST同工酶出现一些新的谱带,这种影响因La3+浓度不同而异。

The effects of different concentrations （0. 01, 0. 1, 2. 0 and 5.0 mg·L-1 ） of La3＋ on the antioxidant activity and esterase （EST） isozymes in kidney and gill of Cyprinus carpio Linnaeus were studied by renewal test. The results indicated that within the test concentration range of LaCl3 and after treated for 4 months, the SOD activities in C. carpio gill was inhibited signifi- cantly, while the SOD activity in kidney and the LPO content in gill had no significant differences with the control. The kidney＇s LPO content at 0. 01,0. 1 and 5.0 mg ·L-1 of La3＋had no sig- nificant difference with the control, but was much higher at 2.0 mg ·L-1of La3＋ The POD activities in kidney and gill at 0. 01 and 0. 1 mg ·L-1 of La3＋ were significantly higher than the control, but were inhibited significantly at 5.0 mg·L-1of La3＋. PAGE test showed that under effects of La3＋ , the band lightness of SOD and EST in kidney and gill changed distinctly, several bands of EST isozymes in kidney and gill disappeared, and some new bands of EST isozymes in kidney were observed. These effects varied with different La3＋ concentrations.