KZY-2复方制剂对小鼠吞噬细胞吞噬功能的影响

Effects of KZY-2 compound on mice phagocytic function

目的：探讨知母等抗真菌中药有效组分复方制剂（KZY-2）的免疫药理作用。方法：（1）采用BALB／c小鼠体内吞噬实验法，设立对照组与试验组，对比观察试验组与对照组小鼠中性粒细胞对白假丝酵母菌标准菌株（ATCC 14053、CCCM Cla）和临床分离菌株（Cl-1、Cl-2、Cl-3、Cl-4）的吞噬百分数及吞噬指数。（2）将不同浓度的药物分别注射至小鼠腹腔，观察药物浓度对吞噬细胞吞噬功能的影响。结果：（1）低浓度药物作用结果：①吞噬百分数均值及P值：对ATCC 14053、CCCM C1a、Cl-1、Cl-2、Cl-3、Cl-4依次为：75．1％、P〈0．01，67．9％、P〈0．01，54．8％、P〈0．05，52．6％、P〈0．05，66．3％、P〈0．05；43．6％、P〉0．05。②吞噬指数均值及P值：依次为2．03、P〈0．01，1．53、P〈0．01；0．873、P〉0．05，0．783、P〉0．05 0．505、P〉0．05，0．356、P〉0．05。（2）高浓度药物作用结果：①吞噬百分数均值及P值：对ATCC 14053、Cl-4依次为：83．21％、P〈0．01：66．5％、P〈0．05。②吞噬指数均值及P值：对ATCC 14053、Cl-4依次为：2．11、P〈0．01；1．23、P〈0．05。结论：KZY-2复方制剂具有明显增强小鼠中性粒细胞对白假丝酵母菌的吞噬作用，吞噬细胞对实验菌吞噬作用的强弱与药物浓度有关。

Objective： To investigate the immune pharmacological action of the anti-fungal drug （ KZY-2 compound）. Methods： （ 1 ） By means of phagocytizing Candida albicans exporimentation in vivo, BALB/c mice were assigned randomly into control group and exporiment group, （PMN） granulocytic porcentage and phagocytic index of two groups were measured;（2）To observe effects of medicine on mice phagocytic function,different concentrations of drug were injected to mice abdominal cavity. Results： （1） When the drug concentration was low, the mean of Phagocytize poreentage of ATCC14053, CCCMC1a, Cl-1, Cl-2, Cl-3 and Cl-4 were respoctivdy 75.1%, 67.9%, 54.8%, 52.6%, 66.3%, 43.6%, the corresponding P value were P〈0.01,P〈0.01 ,P〈0.05,P〈0.05,P〈0.05 and P〉0.05 ; the mean of phagocytic index of ATCC14053, CCCMC1a, Cl-1, Cl-2, Cl-3 and Cl-4 were respectively 2.03, 1.523, 0.873, 0.783, 0.505, 0.356, the corresponding P value were P〈0.05, P〈0.05, P〉0.05, P〉0.05, P〉0.05 and P〉0.05. （2） When the drug concentration was high, the mean of Phagocytize pereemage of ATCC14053 and Cl-4 were respectively 83.2% and 66.5%, the corresponding P value were P 〈0.01 and P 〈0.05 ; the mean of phagocytic index of ATCC14053 and C1-4 were respectively 2.11 and 1.52, the corresponding P value were P 〈 0.01 and P 〈 0.05. Conclusions： KZY-2 compound could enhance phagocytosis of granulocyte on Candida albicans, and the role of intensity was related with the drug concentration.