大孔吸附树脂纯化刺嫩芽皂甙的工艺研究

STUDY ON THE PURIFICATION OF TOTAL SAPONINS FROM ARALIAELATA(MIQ)SEEM WITH MACROPOROUS ADSORPTION RESIN

以辽宁产刺嫩芽为试验材料,通过筛选树脂的种类、研究pH值、流速和洗脱剂浓度对吸附过程的影响,确定出大孔树脂纯化刺嫩芽皂甙的新方法。结果表明:大孔树脂AB-8对刺嫩芽皂甙的吸附量大,解析率高,纯化效果较好;最佳柱纯化条件:洗脱液pH值为8.0、洗脱流速为0.6mL/min、洗脱剂浓度为70%。将洗脱液浓缩,真空干燥即得高纯度刺嫩芽皂甙,纯度达90%以上,为纯化皂甙研究出一种好方法。

We uses Araliaelata （Miq） Seem witch growed in Liaoning province as material.A new method for purification of saponins with macroporous resin was studied.The macroporous resin AB-8 was chosen as the adsorbent. The results showed that the optimum column purification condition was as follows： the aqueous solution of crude extract of Araliaelata （Miq） Seem at pH 8.0, the flowing rate at 0.6 mL/min,the eluting alcohol concentration was 70 %. The eluent of 70 % ethanol was collected, concentrated and freeze---dried, the Araliaelata（Miq） Seem saponins was obtained .The purity of saponins was above 90 %. We developed a good method for saponins. purifying