基因工程技术在醋酸菌改良中的应用

APPLICATION OF GENE TECHNOLOGY ON THE MODIFIED ACETIC ACID BACTERIA

醋酸发酵过程乙醇脱氢酶(ADH)、乙醛脱氢酶(ALDH)酶系起着重要作用,主要影响着醋酸菌的产酸速率和耐酸性,介绍了醋酸菌的耐热机制和耐酸机制。阐述了基因dnaKJ与groESL等基因克隆技术在耐高温醋酸菌构建上的应用,醋酸耐酸因子aarA、aatA以及ADH、ALDH、辅酶Q(PQQ)和不饱和脂肪酸、顺乌头酸酶等对酸度的耐受情况,通过过量表达上述影响因子提高醋酸菌的耐酸性。展望了在国家863等项目引导下研究机构和企业加强醋酸发酵的基础研究以便于构建高活性的醋酸菌。

This review examines the enzymes acid fermentation, particularly on controling such as ADH and ALDH were playing important roles in acetic the productivity and acid tolerance. The recently investigations, more important genes technology were used on getting new modified bacteria. Clone and overexpress the genes responsible for acetic acid thermoresistant and resistance such as dnaKJ/groESL/aarA/ataA and ADH/ALDH as well as PQQ to get highly tolerant acid bacteria. It reports China already paid top dollar on the research of high efficiency vinegar fermentation and built new acetic acid bactera, such as 863 has already started to supportort this project, there will be great improvement in China＇ s vinegar ferment technology and equipment with factories and academy work together.