摘要
本研究应用３０％Ⅲ度烧伤大鼠模型，旨在探讨烧伤早期内皮素变化规律及其在心脏损害中的作用，通过对大鼠烧伤后血浆、心脏内皮素含量动态变化及非选择性内皮素受体拮抗剂ＰＤ１４５０６５对心脏损害防治作用的观察。研究发现：烧伤后血浆及心肌组织内皮素明显增加，且与伤后心脏含水量的改变有一定的相关性；伤后及时应用ＰＤ１４５０６５则可一定程度增加心肌组织ＳＯＤ、Ｎａ＋－Ｋ＋－ＡＴＰ酶活性及ＡＴＰ、ＡＤＰ含量，减少ＭＤＡ、ＡＭＰ含量，减轻组织水肿，光、电镜检查也证实可减轻心脏损害。这些结果表明烧伤后内源性内皮素的增加参与了烧伤早期心脏损害的发生，其机制可能是伤后异常升高的内皮素通过收缩血管造成心肌组织缺血缺氧性损害，继而导致能量代谢紊乱及脂质过氧化损害。
The purpose of this study was to investigate the role of endogenous endothelins in postburn heart injury by use of a 30% TBSA Ⅲ degree burnt rat model. Dynamic changes of endothelins in postburn rats were measured, and the effects of PD 145065 , a kind of non selective endothelin receptor antagonist, on heart injury in burnt rats were observed. The main results were as follows: Not only did the endothelin concentration in plasma of burnt rats elevate significantly, but also the endothelin contents in heart muscle apparently increased, which negatively correlated with the changes of water content in heart. Administration of endothelin receptor antagonist PD 145065 had been morphologically and biochemically demonstrated to be able to prevent heart from damage to some extents, by means of stimulation of the activity of SOD or N +-K + ATPase, enhancement of the contents of ATP and ADP, and reduction of the severity of edema in heart. These results suggest that endogenous endothelin may contribute to the postburn heart injury in the early stages in rats through vascular contraction and tissue hypoxia which in turn induces ATP depletion and lipoperoxidation damage.
出处
《军医进修学院学报》
CAS
1997年第3期175-178,共4页
Academic Journal of Pla Postgraduate Medical School
基金
国家自然科学基金
