非淀粉粘性多糖酶在大麦水解中的应用研究

RESEARCH ON APPLICATION OF NON-STARCH VISCOUS POLYSACCHARIDASE IN ENZYMATIC HYDROLYSIS OF BARLEY

研究了水解大麦中非淀粉粘性多糖酶的酶学性质,采用改良的DNS法测定酶活。结果表明,水解酶A和B的最适作用条件均为pH值6.5,温度50℃;以国产大麦为原料,研究了非淀粉粘性多糖酶在大麦中的应用,结果显示,A和B的最佳添加量分别为2.0U/g原料和3.0U/g原料,β-葡聚糖含量降解率达到92.6%,粘度降低率为31.9%;采用两段式糖化法与一次糖化相比,大麦汁黏度降低率提高了6.2%。

Enzyme properties of non-starch viscous polysaccharidase for hydrolysis of barley was studied. Enzyme activities was determined by using improved DNS method. The result showed that the optimum pH and temperature for hydrolase A and B were pH6.5 and 50℃ respectively; Application of non-starch wiscous polysaccharidase in enzymatic hydrolusis of barley was researched,used domestic barley as raw material.It was found that the optimal addition for A and B were 2.0 U/g and 3.0 U/g raw materials respectively by application on enzymatic hydrolysis of barley, the degradation rate of β-glucan content reached to 92.6 %, and the reduced rate of barley wort viscosity was 31.9 %;Compared with non-step saccharification, using two-step saccharification the rate of barley wort viscosity reduction was improved by 6.2 %.