基于灰度的微血管通透性图像分析

Image analysis of the wall permeability of microvessels based on image gray

目的：建立肠系膜微血管的通透方程。方法：应用微循环活体观察和荧光示踪技术，通过计算机数字图像处理和自行研制的图像分析软件对荧光素钠（ＦｌＮａ）在肠系膜微血管的通透过程进行定量研究。结果：在注射荧光物质后早期的通透速度较快，当微血管周围组织中的荧光物质达到一定浓度后，通透速度明显减慢。通透过程呈两段式线性分布，每一时段内的通透速度恒定，第二时段的通透速度比第一时段慢约５倍。通透方程：ｔ＜２０ｓ：ｘ＝－３．４０６９＋６．０４５４×ｔ；ｔ＞２０ｓ：ｘ＝９９．４４５１＋１．０６２４×ｔ，通透速度：ｔ＜２０ｓ：Ｄ＝１．１６３５×１０－７ｃｍ２／ｓ；ｔ＞２０ｓ：Ｄ＝０．２０４５×１０－７ｃｍ２／ｓ。结论：本方法为进一步建立不同分子量的通透方程及通透速度与分子量的关系，以及定量研究体外培养的单层内皮细胞的通透性，从而达到全面、定量地测试及评价微循环物质交换参数的目的，提供了数学基础。

Objective:An intravital fluorescence microscopic method and digital image analysis were established for measuring the changes of fluorescein sodium (FlNa, MW 376) permeability in mesenterial microvessels. Methods:The transport of fluorescent tracer within the blood vessels and through the vessel walls into the perivascular tissue of the rat mesentery was study using a highly sensitive CCD camera, the fluorescence intensities were transformed into video signals and recorded for subsequent analysis. Results:The results show that FlNa can pass through the wall of microvessels with minimal delay. The permeabiity velocity was a constant (1.1635×10 -7 cm 2/s) and very fast within the first 20 secend, after that,the velocity was 5 times slower and still a constant (0.2045×10 -7 cm 2/s).Conclusion:it is expected that this method would be very useful for the quantitive analysis in various molecular weights and in monolayer endothelial cells permeability.