结核病患者血液中抗原-抗体复合物的测定及临床意义

Detection of the antigen antibody complexes in blood of the patients with tuberculosis and its clinic significance

目的：调查结核病患者血液中抗原－抗体循环免疫复合物（ＣＩＣ）含量和特异性，了解其临床意义。方法：聚乙二醇沉淀比浊法检测血清中ＣＩＣ；ＥＬＩＳＡ法测定血清和ＣＩＣ中抗结核抗体。结果：结核病患者ＣＩＣ水平显著高于正常人（Ｐ＜０．０１）；结核病患者ＣＩＣ中抗结核抗体水平也显著高于正常人（Ｐ＜０．０１）；ＣＩＣ阳性者均为活动性肺结核患者。

Objective:The aim of this paper was to investigate the quantity and specificity of antigen antibody circulatory immune complexes (CIC) in the serum of tuberculosis patients. Methods:The cirrculating immune complexes were performed by precipitation tests using polyethyleneglycol (PEG) on seres of normal and pathological sera; and the antituberculous antibodies in the serum and CIC were detected with enzyme linked immunosorbent assay (ELISA). Results:The quantity of CIC in the serum of patients with tuberculosis was significantly higher than that of healthy people. The level of the antituberculous antibodies in the CIC of the patients with tuberculosis was also significantly higher than that of healthy people. Conclusion:The detection of CIC in the serum of patients with tuberculosis may offer a high specificity in the diagnosis of active pulmonary tuberculosis.