雷公藤多甙对大鼠急性肝衰竭的保护作用

Protective effect of glycoside of Tripterygium wilfordii on acute experimental hepatic failure in rats

目的探讨雷公藤多甙（ＧＴＷ）对实验性肝衰竭的保护作用．方法雄性Ｗｉｓｔａｒ大鼠２４只，随机分为对照组（ｎ＝８），急性肝衰竭（ＡＨＦ）组（ｎ＝８）和ＧＴＷ保护组（ｎ＝８）．ＧＴＷ组在实验前５ｄＧＴＷ２５ｍｇ／（ｋｇ·ｄ）经胃管灌胃，其余两组均以等量生理盐水溶液灌胃．从第６天开始，ＡＨＦ组和ＧＴＷ组均在空腹１２ｈ后ｉｐＤＧａｌＮ１６ｇ／ｋｇ，注射后４０ｈ，以３０ｇ／Ｌ戊巴比妥钠（４０ｍｇ／ｋｇ）ｉｐ麻醉，心脏抽血测定血清ＡＬＴ，ＴＢ和Ｔ淋巴细胞亚群．同时，在光镜和电镜下观察各组肝组织病理变化．结果ＡＨＦ组ＡＬＴ（ＩＵ／Ｌ）和ＴＢ（μｍｏｌ／Ｌ）分别为７８２８±５７６２和１２５６２７±６７０２７；ＯＸ８水平为１４０％±３％．ＧＴＷ组ＡＬＴ和ＴＢ分别为３５９±５４和４７±３５；ＯＸ８为４３％±４％．两组比较有显著统计学差异（Ｐ＜００５或００１）．同时，前者电镜下线粒体和内质网肿胀、破损，核内染色质凝聚，后者细胞器受损明显减轻．结论雷公藤多甙对实验性急性肝衰竭具有保护作用．

IM To evaluate the protective effect of glycoside of Tripterygium wilfordii (GTW) on experimental acute hepatic failure (AHF). METHODS Twentyfour Wistar rats were randomly assigned to three groups: control group (n=8), AHF group and GTW protection group, GTW of 25mg/(kg·d) was given ig for 5 days to the GTWprotection group, while only normal saline in equal amount was given to the other groups for 5 days. On the 6th day, AHF and GTW groups were respectively administered with DGalN of 16g/kg, ip after 12 hours fasting. Fourty hours later 30g/L natrii pentobarbitalum (40mg/kg) was given ip for anesthesia. Blood was drawn from the hearts to measure serum ALT, TB and Tcell subsets. The hepatic lesions were evaluated by light and electron microscopy. RESULTS Serum amylase (IU/L) and TB levels (μmol/L) were much lower, OX8 cells much higher in GTW group than those in AHF group (359±54 vs 783±58, 47±35 vs 126±67, P<001; 43%±4% vs 14%±3%, P<005). Mitochondria swelled and injured, enchromatin highly aggregated in the former, but those in the latter were almost normal. CONCLUSION GTW has a protective action on AHF.