外周血TNF,sIL-2R含量及淋巴细胞亚群变化与慢性乙型肝炎肝组织病变的关系

Relationship between peripheral TNF and IL2R concentrations and lymphocyte subpopulations and liver lesions

目的动态观察促肝细胞生长素（ｐＨＧＦ）对慢性乙型肝炎（以下称慢性乙肝）患者血清肿瘤坏死因子（ＴＮＦ），可溶性白介素２受体（ｓＩＬ２Ｒ）含量及外周血淋巴细胞亚群比例的作用，并观察ＴＮＦ，ｓＩＬ２Ｒ含量及淋巴细胞亚群的比例与慢性乙肝肝组织病变的关系．方法收集５８例血清ＡＬＴ反复高于正常，肝炎病史及血清ＨＢＶＭ阳性１ａ以上的慢性乙肝患者与２次肝组织活检同步的外周血，用ＥＬＩＳＡ法检测血清ＴＮＦ，ｓＩＬ２Ｒ含量，ＡＰＡＡＰ法检测外周血淋巴细胞亚群．结果治疗后，慢性乙肝重型血清ＴＮＦ及ｓＩＬ２Ｒ的含量分别为５８ｍｇ／Ｌ和４９３８ｋＵ／Ｌ，ＣＤ４＋为４２８％，ＣＤ８＋为２４３％，ＣＤ４＋／ＣＤ８＋为１７２；重型伴活动性肝硬变血清ＴＮＦ及ｓＩＬ２Ｒ含量分别为９２ｍｇ／Ｌ和６６９７ｋＵ／Ｌ，ＣＤ４＋为４１２，ＣＤ８＋为２５３，ＣＤ４＋／ＣＤ８＋为１６３，均较治疗前有显著改善（Ｐ＜００１）．同时肝组织病变明显改善．说明血清ＴＮＦ，ｓＩＬ２Ｒ含量及外周血淋巴细胞亚群的比例与慢性乙肝的肝组织病变之间存在着动态关系．结论血清ＴＮＦ，ｓＩＬ２Ｒ含量及外周血淋巴细胞亚群的比例与慢性乙肝的肝组织病变?

IM To observe the effects of hepatocyte growth factor (HGF) on peripheral blood TNF and IL2R concentrations and the ratio of lymphocyte subpopulations, and study their dynamic relationship with liver tissue lesions of chronic hepatitis B. METHODS A total of 58 patients received 80mg pHGF i.v. once a day for 3 months. Before and after treatment, patients received two liver biopsy examinations, at the same time, the peripheral TNF and IL2R concentrations and the ratio of lymphocyte subpopulations were detected by ELISA and APAAP methods, respectively. RESULTS There was a statistically significant difference in the peripheral TNF and IL2R concentrations, and the ratio of lymphocyte subpopulations before (severe type and severe type with active cirrhosis: 102,6856;331,327 and 146,8269;317,339) and after treatment (severe type and severe type with active cirrhosis: 58,4938;428,243 and 92,6697;412,253,P<001). Liver tissue lesions were improved markedly. CONCLUSION Peripheral blood TNF and IL2R concentrations and the ratio of lymphocyte subpopulations are related with the liver tissue lesions of chronic hepatitis B.