HBV侵犯PBMC致线粒体功能改变

Mitochondrial function changes of peripheral blood mononuclear cells infected with HBV

目的探讨ＨＢＶ侵犯外周血单个核细胞（ＰＢＭＣ）后对其线粒体功能的影响．方法ＨＢｓＡｇ阳性６个月以上，无肝炎症状及体征，肝功正常患者５８例．多聚酶链反应检测ＰＢＭＣ中ＨＢＶＤＮＡ，噻唑兰（ＭＴＴ）比色法测线粒体功能．ＨＢｓＡｇ、ＨＢｅＡｇ检测用固相放免法检测．结果慢性ＨＢＶ感染者５８例，ＰＢＭＣ中检出ＨＢＶＤＮＡ３１例（５３５％），ＰＢＭＣ中ＨＢＶＤＮＡ阳性组ＭＴＴ比色法查线粒体功能的Ａ（ＯＤ５００ｎｍ）值明显低于ＨＢＶＤＮＡ阴性组（００５±００３ｖｓ０２９±００７，Ｐ＜００１）．结论慢性ＨＢＶ感染时，ＨＢＶ常侵犯ＰＢＭＣ，并导致ＰＢＭＣ线粒体功能降低、能量代谢异常．这可为ＨＢＶ慢性感染免疫功能异常的原因之一．

IM To investigate the changes of mitochondrial function of peripheral blood mononuclear cells (PBMC) infected with HBV. METHODS Polymerase chain reaction was used to detect HBV DNA in PBMC and the MTT chromatometry was used to determine the mitochondrial function. HBV serum markers, IgM antiHAV, antiHCV were tested with radioimmunoassay. RESULTS Of 58 patients with chronic HBV infection, 31 (535%) were positive for HBV DNA in PBMC. The mitochondrial function A(OD 500nm) of HBV DNA positive PBMC (005±003) was much lower than that in HBV DNA negative PBMC (029±007) (P<001).CONCLUSION In chronic HBV infection patients, PBMCs were often infected with HBV, leading to lowered mitochondrial function. This may be one of the reasons for disturbance of immune function in patients with chronic HBV infection.