KCNJ11基因多态性与2型糖尿病的关联研究

Association Analyses of KCNJ11 Gene Polymorphisms with Type 2 Diabetes Mellitus

目的:探讨β-细胞腺苷三磷酸-敏感性钾通道(potassium inwardly-rectifying channel,sub-family J,member 11,KCNJ11)基因E23K多态性与湖北汉族人群2型糖尿病的相关性。方法:采用同胞对(家系内对照)和随机病例-对照两种实验设计,利用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术分析湖北地区443例样本KCNJ11基因E23K多态性,并测定身高、体重、腰围、臀围、血压和空腹血糖等生理生化指标。结果:随机病例-对照实验设计中,病例组与对照组的基因型频率与等位基因频率有显著性差异(P﹤0.05或P﹤0.01)。结论:在湖北汉族人群中,2型糖尿病的发生发展与KCNJ11基因E23K多态性相关联,KCNJ11基因是湖北汉族人的一个2型糖尿病易感基因。

Objective： To study the correlation between KCNJ11 E23K polymorphism and T2DM in Han Chinese in Hubei. Methods： Both sib-pairs and random case-control designs were used, the genotypes of KCNJ11 gene were analyzed by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） in 443 samples. Height, weight, blood pressure and fasting blood glucose were measured. Results： There were significant differences in the genotypie and allelic frequencies of the KCNJ11 gene E23K polymorphism between cases and controls in random case-control design （P 〈 0.05 or P 〈 0.01 ）. Conclusion： The KCNJ11 gene E23K polymorphism is associated with T2DM and may be a susceptibility locus for T2DM in Han Chinese in Hubei.