离子束注入对细胞有丝分裂微管骨架影响的研究

Study on effect of Microtubule Skeleton in cell Mitosis by ION Bean Implantation

经能量为２０～３０Ｋｅｖ注入剂量为６×１０１５Ｎ＋／ｃｍ２和１０×１０１５Ｎ＋／ｃｍ２的Ｎ＋离子注入及免疫荧光抗体标记显微观察和统计，结果表明，在绿豆根尖细胞有丝分裂中，分裂细胞的形体、中后期细胞数目、细胞微管骨架以及纺缍体的排布方式均发生了异常变化。１２－１３％的分裂细胞个体增大，８－１０％有丝分裂中后期细胞数目高于对照组，间期细胞微管骨架荧光强度明显弱于对照组，且有２－４％的较强荧光小体和无荧光小体（微孔洞，ｍｉｃｒｏｏｐｅｎｉｎｇ）。３－１２％的中后期细胞中出现不对称和极向不同步移动排布的纺缍体。研究结果表明：２０～３０Ｋｅｖ的能量和剂量为６～１０×１０１５Ｎ＋／ｃｍ２的Ｎ＋离子束注入，对绿豆根尖细胞有丝分裂微管骨架可诱发产生异常变化，同时也可以初步认为这种变化结果与Ｎ＋离子束注入产生的染色体异常分配和运动功能活动有密切的相关性。

After implanting N + ion beam with 20～30Kev into seed of Phaseolus aureus and detecting by immunofluorescence micrographic cytochemistry,the tesult showed that some abnormal phenomena in the cell of root-tip during cell mitosis phase,12%-13% cell volume expanded,the number of the cell in mitosis anaphase increased by 8%-10% compared with ck:2%-4% fluorescenceosome and micro-opening appeared,asymmetric spindle was observed in about 3%-12% interphase and anaphase cell,these results showed that implanting dose 6～10×10 15 N +/cm 2 ion beam with 20 Kev could induce abnormal microtubtle-skeleton in the mitosis of Phaseolus aureus root-tip cell,meanwhile it also showed that these changes were related with the chromosome abnormal distrubution and movement resulted from implanting N + ion-beam.