左手螺旋Z-DNA的研究 Ⅴ 环境因子对Oligo-d(G-C)_6构象的影响

Studies on Z-DNA Ⅴ.Effects of Environmental Elements on The Conformation of Oligo-d(G-C) 6 *

脱氧寡聚核苷酸ｄ（Ｇ－Ｃ）６是迄今所报道的同类脱氧多聚核苷酸中能形成左手螺旋ＤＮＡ（Ｚ－ＤＮＡ）的最短序列。环境因子如ＰＨ，温度和离子的类别与浓度对ｄ（Ｇ－Ｃ）６在溶液中的Ｚ－构象的形成和Ｂ－、Ｚ－构象的相对稳定性有着明显的影响。在合适的条件下，ｄ（Ｇ－Ｃ）６在溶液中可呈现Ｂ－构象区，Ｚ－构象相对稳定区和Ｂ－、Ｚ－构象跃迁过渡区。

Based on literature search,Oligo-d(G-C) 6 is the shortest sequence for the same type of oligonucleotides which can be induced to transition from B-DNA to Z-DNA. The effects of enviromental elements such as pH,temperature and especially, kinds and concentrations of inorganic salts on the B-Z conformation of Oligo-d(G-C) 6 were studied.