氮离子束注入大麦种子的细胞生物学效应

Cytological Effects of N + Ion Beam Implantation on Barley Seeds

本文研究了用３０ＫｅｖＮ＋离子束注入大麦干种子后其Ｍ１代的细胞生物学效应。研究结果表明，低剂量的Ｎ＋离子注入对大麦种子的萌发及Ｍ１胚根、胚芽细胞的有丝分裂有明显的促进作用。离子注入均能诱发胚根细胞和胚芽细胞的染色体畸变和核畸变，呈现微核、双核、小核、桥、断片和落后染色体等多种类型。并在２×１０１６Ｎ＋／ｃｍ２－８×１０１６Ｎ＋／ｃｍ２剂量范围内，注入剂量与畸变率之间有显著的正相关，但到１×１０１７Ｎ＋／ｃｍ２后畸变率却反而下降。研究结果还显示胚芽细胞较胚根细胞对氮离子束更为敏感。

After implanting of N + ion beam with 30 kev into dry seeds of barley, cytological effects in the M 1 generation have been studied in the present paper. The results showed that there was obvious promotion to germination ability of barley seed and mitosis of radicle cells and plumule cells in M 1 generation by low dose implantation of N + ion. N + ion implantation not only could induce chromosomal aberration and nuclear aberration in radicle cells, but also in plumule cells.Many aberration types, such as micronuclei, doublenuclei,minornuclei,chromosomal bridges, fragments, lagged chromosomes and so on were found. And in the range of 2×10 16 -8×10 16 N +/cm 2 ion implantation dose was positively correlated with aberration frequency significantly. However,the dose increased to 1×10 17 N +/cm 2, aberration frequency began to decrease gradually. Experimental reaults also indicated that plumule cells were more sensitive to N + ion than radicle cells.