肺炎衣原体HEP-2培养分离及其抗体的MIF研究

Isolation and Detection of Chlamydia Pneumonia In Fresh Clinical Specimens Respectively With HEP-2 Culture and Microimmunofluorescence Test

肺炎衣原体是一种引起肺炎及呼吸道感染等的新型病原体。本研究采用ＨＥＰ２培养从８例呼吸道感染者新鲜痰液中初步分离出肺炎衣原体。此外，采用微量免疫荧光试验（ＭＩＦ）对９６例呼吸道感染者与４８例健康献血员的血清学分析表明：８３．４％的患者肺炎衣原体ＩｇＧ抗体阳性，与对照组比较，统计学上有非常显著差异。提示这些患者呼吸道感染中，肺炎衣原体可能起较大作用。

Chlamydia pneumoniae is a new species of pathogen causing pneumonia and respiratory tract infection.C.pneumonia has been isolated primarily from eight patients with respiratory tract infection in our study with HEP2 culture.In addition,sera from 96 patients and 48 healthy blood donors have been assayed with MIF test.The results showed that C.pneumonia IgG antibody of 83.4%patients was positive,demonstrating very significant statistical differences if compared with the control and suggesting C.pneumonia infection may be the major cause for the patients' respiratory infection.