光敏核不育水稻农垦58S与正常品种“农垦58”在pmsl区段无育性基因分离

PMSl IS NOT THE LOCUS RELEVANT TO FERTILITY DIFFERENCE BETWEEN THE PHOTOPERIOD-SENSITIVE MALE STERILE RICE NONGKEN 58S AND NORMAL RICE “NONGKEN 58”

光敏核不育水稻农垦58S系由正常品种“农垦58”(Oryza sativa L.ssp.japonica)自然突变产生。为弄清该突变基因在染色体上的位置,曾用覆盖整个水稻基因组的300余个RFLP探针对农垦58S和“农垦58”进行了对比分析,得到了7个具多态性的探针,其中2个探针RG30和RZ626正好落在第7染色体上以前定位的光敏核不育基因pmsl所在的区段。以这两个标记对农垦58S/“农垦58”组合F_2随机群体140单株进行了RFLP分析,按RFLP基因型分组对育性作方差分析,结果表明,这2个标记位点与此群体中引起育性分离的位点无连锁关系。说明由正常“农垦58”变为光敏核不育农垦58S的突变基因不在pmsl区段。

The photoperiod-sensitive male sterile rice, Nongken 58S,was obtained as a spontaneous mutant of the Oryza saliva L. ssp. japonica cultivar "Nongken 58" . To determine the chromosomal location of the locus related to the fertility difference between Nongken 58S and its wild-type ancestor , the authors assayed the DNA polymorphisms between these two varieties using a total of over 300 RFLP probes covering the entire molecular marker linkage map. Seven probes detected polymorphisms between "Nongken 58" and Nongken 58S. Two probes,RG30 and RZ626, both from chromosome 7, happened to be located in the genomic region of pmsl, a locus for photoperiod-sensitive male sterility identified in the authors' previous study. These two probes were used to assay a random sample of 140 individuals from a F2 population of a cross between Nongken 58S and "Nongken 58", in which the fertility segregated in a typical 3:1 ratio. An analysis of variance of the fertility using the RFLP genotypes as the groups clearly evidenced that these two marker loci are not linked to the locus associated fertility segregation in this population. It is concluded that the locus relevant to fertility difference between Nongken 58S and "Nongken 58" is not in the vicinity of the pmsl region.