摘要
A 508 bp fragment of a putative pheromone receptor gene from Lentinula edodes was amplified by PCR using a pair of degenerate primers designed according to conserved sequences of pheromone receptor genes reported in other Basidiomycetes.The fragment encodes 117 amino acids and contains three introns,56 bp,51 bp and 50 bp in size.Alignment analysis revealed that the deduced amino acid sequence of the L.edodes fragment exhibited 65% identity and 82% similarity with the corresponding segments of pheromone receptors reported in Coprinus cinereus and Schizophyllum commune.
A 508 bp fragment of a putative pheromone receptor gene from Lentinula edodes was amplified by PCR using a pair of degenerate primers designed according to conserved sequences of pheromone receptor genes reported in other Basidiomycetes.The fragment encodes 117 amino acids and contains three introns,56 bp,51 bp and 50 bp in size.Alignment analysis revealed that the deduced amino acid sequence of the L.edodes fragment exhibited 65% identity and 82% similarity with the corresponding segments of pheromone receptors reported in Coprinus cinereus and Schizophyllum commune.
出处
《食用菌学报》
2008年第2期4-6,共3页
Acta Edulis Fungi
基金
the Foundation of Shanghai Agaricultural Committee(No.2003-4-5)
Foundation of Shanghai Academy of Agricultural sciences(2007-24 and 2007-07)
关键词
克隆技术
信息素
受体
基因
Lentinula edodes
pheromone receptor
degenerate PCR
