食用油烟对大鼠体内脂质过氧化反应的影响

The Effects of Lipid Peroxidation on Rats Induced by Cooking Oil Vapors Inhalation

本研究采用动式染毒技术，将大鼠分成4个浓度组吸入豆油油烟（油温220℃，观察血中SOD，GSH，MDA含量，肺组织中LDH，ACP，MDA和肺巨噬细胞（PAM）膜脂流动性（LFU）等指标的变化，探讨吸入油烟的急性毒作用机理。实验结果表明：油烟平均浓度为1．05mg／m3时，血浆及肺组织中MDA开始增加（P＜0.05），且含量随吸入油烟量增加；平均浓度为9．58mg／m3时，全血SOD活力明显降低（P＜0．05）且伴有GSH的下降，同时肺泡灌洗液中PAM膜脂流动性增加（P<0．01）且LDH，ACP酶活力升高（P＜0.05）。提示急性吸入豆油油烟时在机体及肺组织中可能产生大量自由基，进一步诱发脂质过氧化反应引起肺组织细胞的破坏，崩解，导致以肺急性炎症为主的机体损伤反应。

A dynamic inhalant intoxication technique has been applied to reveal the effects of the soybean oil vapors on adult rats: After exposure to the various oil vapors concentration (i,e,0,1.05, 2.72, 5.50, 9.58 mg/m3 respectively) for 2 hours, the results demonstrated that (1) Malondiadehyde (MDA) contents in plasma and in lung hssue increased gradually with the oil concentration and significantly raised in lung tissue at lower treated group (i.e. 1.05 mg/m3)compared with the contrast group (p<0.05); while the glutathione-SH (GSH) and superoxide dismutase (SOD) decreased with the concentration and the changes later than the MDA. (2) At the high treated group (9.58mg/m3, the membrane lipid fluidity (LFU) of the pulmonary alveolar macrophages (PAM) in bronchoalveolar lavage fluid (BALF) increased (p<0.01), compared with the activities of lactate dehydrogenase (LDH) and acid phosphatase (ACP) in lung tissue (p<0.05).These findings suggested that acute inhalation of soybean vapors may produce lipid peroxidation and then lead to damage dominatedly in lung tissues, the injury increased with the oil vapors inhalation.