PPAR-γ2 Pro12Ala基因多态性与2型糖尿病肥胖的相关性研究

Correlation of PPAR-γ2 Pro12Ala Gene Polymorphism with Type II Diabetes Mellitus Obesity

目的探讨PPAR-γ2 Pro12Ala(P12A)基因多态性(包括PP、PA、AA3种基因型,用X/A作为PA、AA共同代称)与2型糖尿病(T2DM)肥胖是否相关。方法根据体质指数(BMI)将228例T2DM患者分为肥胖组(BMI>25 kg/m2,105例)和非肥胖组(BMI≤25 kg/m2,123例)。应用聚合酶链反应-限制性片段长度多态性分析(RFLP-PCR)进行基因型测定。比较两组患者PPAR-γ2 P12A基因突变率、等位基因分布情况及临床生化指标。分析PPAR-γ2 P12A基因与T2DM肥胖的相关性。结果T2DM患者中,肥胖组与非肥胖组患者PPAR-γ2 P12A基因突变率及等位基因分布间差别无统计学意义(P>0.05)。T2DM非肥胖组X/A基因型者,血清三酰甘油、高密度脂蛋白水平及尿清蛋白/肌酐与PP基因型者比较,差别有统计学意义(P<0.05)。PPAR-γ2 P12A基因多态性与肥胖的发生无相关性〔OR=1.639,95%CI(0.832,3.233)〕。结论T2DM患者中PPAR-γ2 P12A基因多态性与T2DM患者肥胖的发生无关。

Objective To find out whether PPAR - γ2 Pro12Ala （P12A） gene polymorphism （including PP, PA and AA genetypes, using X/A as common name for PA and AA） is correlated with type 2 diabetes mellitus （T2DM） obesity. Methods According to body mass index （BMI）, 228 T2DM patients were divided into obese group （ BMI 〉 25 kg/m^2, n = 105 cases） and non -obese group （BMI≤25 kg/m^2, n = 123 cases）. We determined the genotypes by polymerase chain reaction - restriction fragment length polymorphism （RFLP -PRC）, compared PPAR - γ2 P12A gene mutation rate, distribution of alleles and clinical biochemical indicators, and analyzed the correlation of PPAR - γ2 P12A gene with obesity. Results PPAR - γ2 P12A polymorphism might not be an independent genetic risk factor of obesity in T2DM patients [ OR = 1. 639, 95% CI （0. 832, 3.233 ） ] ; non - obese group carried Ala allele, TG A/C were significantly higher and HDL significantly lower in them than in those carrying PP allele. Conclusion PPAR - γ2 P12A gene polymorphism, which may not be an independent genetic risk, is not correlated with Obesity in T2DM patients.