粉刺侧孢霉产木质素过氧化物酶酶活性与pH值的关系

Relationship between lignin peroxidase activity of Phanerochaete chrysosporium and pH value.

研究了粉刺侧孢霉 (Phanerochaetechrysosporium)在以微晶纤维素为C源时 ,木素过氧化酶与 pH值的关系 .试验采用 37～ 39°C下液体振荡培养 ,藜芦醇为产酶诱导剂 ,测试不同起始 pH值所得木素过氧化物酶活性 .结果表明 ,起始 pH值 6.0所得酶活最高达 0 .1 2 6U·ml- 1 ,说明以微晶纤维素代替葡萄糖为C源 ,其最佳 pH值相对较高 .菌丝球直径与酶活呈正相关 .

Phanerochatet chrysosporium was liquid cultured with avicel as carbon source and with veratric alcohol as enzyme inducing agent.Determinations of its lignin peroxidase activity at different initial pH values showed that the highest enzyme activity was obtained at pH 6.0, indicating that the optimum pH value was relatively higher when avicel was used to substitute glucose as a carbon source.The enzyme activity had a positive relationship with the diameter of hyphal pellet.