鸡肌球蛋白轻链激酶表达载体的构建

Construction of Chicken Myosin Light Chain Kinase Expression Vector

鸡肌球蛋白轻链激酶（ＭＬＣＫ）在调节平滑肌细胞收缩中具有重要作用．用ＰＣＲ产生构建所需的限制性内切酶ＳａｌⅠ位点，将鸡ＭＬＣＫｃＤＮＡ插入质粒ｐＢＫｒｓｖ中，构建成ｐＢＫｒｓｖ－ＭＬＣＫ，并通过酶切图谱、ＳａｌⅠ位点序列分析得到证实．重组质粒在大肠杆菌中获得表达．

A MLCK cDNA fragment containing SalⅠ site was generated by Polymerase Chain Reaction (PCR).The pBKrsv MLCK was constructed by inserting MLCK cDNA into pBKrsv polylinker and identified by restriction mapping and sequence analysis of SalⅠ site.These results provides fundation for further investigation of MLCK expression.