槲皮素对完整HL-60细胞中肌醇磷脂转换的抑制作用

Inhibitory effect of quercetin on the turnover of phosphoinositides in intact HL 60 cells

槲皮素对完整ＨＬ┐６０细胞中肌醇磷脂转换的抑制作用康铁邦梁念慈（广东医学院医用生化研究所，湛江５２４０２３）肌醇磷脂信使系统在生物信号的跨膜传递方面起重要作用，并与细胞增殖及肿瘤形成有密切联系［１～５］，有报道：肿瘤细胞或组织中磷脂酰肌醇４－激酶（Ｐ...

Phosphoinositides were extracted by organic solvent and separated by thin layer chromatography(TLC),then were visualized by autoradiography The results showed that quercetin strongly inhibited the proliferation of HL 60 cells,its IC 50 was 46 6 μmol/L after 24 hours treatment and that quercetin could markedly inhibit in a dose dependent manner the turnover of phosphoinositides in intact HL 60 cells This may be one of important mechanisms,by which quercetin inhibits the proliferation and growth of tumor cells.