环六亚甲基双乙酰胺对胃癌不同细胞周期两条信号系统的调节

Effects of HMBA on cAMP-PKA and DAG-PKC Signal Pathways in the Cell Cycle of MGc80-3 Cells

环六亚甲基双乙酰胺 (HMBA)对MGc80 3不同时相细胞内cAMP PKA与DAG PKC两大系统不仅具有正负调控作用 ,而且其作用具有周期特异性 .其中G1期是最敏感的调控时相 ,与对照组相比 ,cAMP水平上升 1 0 2 3% ,PKA活性升高 34 8% ,DAG含量下降 51 4 % ,PKC活性降低 32 3% ;次敏感时相为G2期 ;M期基本没受影响 ;S期变化规律不同于其他时相 .

Modulation of HMBA on cAMP-PKA and DAG-PKC signal pathways in the cell cycle of MGc80-3 cells showed that: 1there were positive and negative regulation between them; 2signal regulation was different in the cell cycle. G1 phase was the most sensitive one among the four phases, cAMP level and PKA activity were increased by 102% and 348%, respectively, as compared with the control; while DAG content and PKC activity were decreased by 5145 and 323%, respectively, as against the control. G2 phase was the second sensitive phase. Effects of HMBA on the signal regulation in M phase were not found. Changes of signal transduction in S phase were different to others.