鼠脑微透析液痕量氨基酸的激光诱导荧光检测

Laser-Induced Fluorescence Detection of Trace Amino Acids in Rat Brain Microdialysates

使用自制微透析探针和活动体位生化取样装置以及自行组装的毛细管电泳 增强型电荷耦合器件 激光诱导荧光系统 ,对鼠脑透析液中的痕量氨基酸以异硫氢酸荧光黄 (FITC)进行柱前衍生后进行了分离和检测 .鼠脑海马CA3区微透析液中游离氨基酸的浓度为 1 0 - 8～ 1 0 - 6mol/L ,并将其用于学习与记忆的研究 ,为无损伤研究活体脑内神经递质和其他痕量生化物质的动态变化提供了一种新方法 .

Trace amount of free amino acids in brain microdialysates was separated and determinated with derivative of fluorescein isothiocyanate (FITC) using the home-made microdialysis probes and biochemical sampling unit for living animal as well as capillary electrophoresis system with intensified charge-coupled device(ICCD)-laser induced fluorescence detector. The concentration of free amino acids in microdialysates was 10 -8～10 -6 mol/L with 5 μl sample. The change of free amino acids was monitored in vivo in the hippocampus of freely moving rats before and after operational learning. These experiments suggest that the sampling and determination system can be used for analysis of amino acids in brain microdialysates.